Prospective evaluation of a novel multiplex real-time PCR assay for detection of fifteen respiratory pathogens-Duration of symptoms significantly affects detection rate
Identifieur interne : 000016 ( PascalFrancis/Checkpoint ); précédent : 000015; suivant : 000017Prospective evaluation of a novel multiplex real-time PCR assay for detection of fifteen respiratory pathogens-Duration of symptoms significantly affects detection rate
Auteurs : Robin Brittain-Long [Suède] ; Johan Westin [Suède] ; Sigvard Olofsson [Suède] ; Magnus Lindh [Suède] ; Lars-Magnus Andersson [Suède]Source :
- Journal of clinical virology [ 1386-6532 ] ; 2010.
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Abstract
Background: Nucleic acid amplification techniques have improved the diagnostic possibilities in respiratory tract infections, although their clinical applicability is not yet fully defined. We have evaluated a multiplex real-time PCR method for the detection of 13 respiratory viruses and 2 bacteria (Mycoplasma and Chlamydophila) in a clinical setting. Objectives: The aim of the present study was to evaluate the diagnostic performance and clinical use of a novel multiplex PCR method in adults with community-acquired respiratory viral infection, and the impact of duration of symptoms on detection rates. Study design: Nasopharyngeal swab samples were prospectively collected from 209 adult outpatients with respiratory infections and 100 asymptomatic controls. Results: An infectious agent was identified in 43% of samples from patients and 2% of asymptomatic controls. The detection rate was significantly higher in samples from patients with a duration of symptoms of 6 days or less (51 %) than in samples from patients with a duration of symptoms of 7 days or more (30%, p < 0.01). For human corona viruses, and influenza virus A and B there was a correlation between the amount of virus in each patient sample as measured Ct values and duration of symptoms. Conclusions: Duration of symptoms significantly affects the detection rate of respiratory pathogens by multiplex real-time PCR in nasopharyngeal swab samples from adult patients with respiratory infections. Our finding should be taken into account when using these tests in clinical practise.
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aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Suède</country><wicri:noRegion>416 85 Gothenburg</wicri:noRegion></affiliation></author><author><name sortKey="Westin, Johan" sort="Westin, Johan" uniqKey="Westin J" first="Johan" last="Westin">Johan Westin</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Clinical Virology, Sahlgrenska University Hospital</s1><s2>Gothenburg</s2><s3>SWE</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Suède</country><wicri:noRegion>Gothenburg</wicri:noRegion></affiliation></author><author><name sortKey="Olofsson, Sigvard" sort="Olofsson, Sigvard" uniqKey="Olofsson S" first="Sigvard" last="Olofsson">Sigvard Olofsson</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Clinical Virology, Sahlgrenska University Hospital</s1><s2>Gothenburg</s2><s3>SWE</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Suède</country><wicri:noRegion>Gothenburg</wicri:noRegion></affiliation></author><author><name sortKey="Lindh, Magnus" sort="Lindh, Magnus" uniqKey="Lindh M" first="Magnus" last="Lindh">Magnus Lindh</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Clinical Virology, Sahlgrenska University Hospital</s1><s2>Gothenburg</s2><s3>SWE</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Suède</country><wicri:noRegion>Gothenburg</wicri:noRegion></affiliation></author><author><name sortKey="Andersson, Lars Magnus" sort="Andersson, Lars Magnus" uniqKey="Andersson L" first="Lars-Magnus" last="Andersson">Lars-Magnus Andersson</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Infectious Diseases, Sahlgrenska University Hospital, Östra</s1><s2>416 85 Gothenburg</s2><s3>SWE</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Suède</country><wicri:noRegion>416 85 Gothenburg</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Clinical Virology, Sahlgrenska University Hospital</s1><s2>Gothenburg</s2><s3>SWE</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Suède</country><wicri:noRegion>Gothenburg</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of clinical virology</title><title level="j" type="abbreviated">J. clin. virol.</title><idno type="ISSN">1386-6532</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of clinical virology</title><title level="j" type="abbreviated">J. clin. virol.</title><idno type="ISSN">1386-6532</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Detection</term><term>Multiplex polymerase chain reaction</term><term>Real time</term><term>Respiratory disease</term><term>Respiratory tract</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Réaction chaîne polymérase multiplex</term><term>Temps réel</term><term>Détection</term><term>Voie respiratoire</term><term>Pathologie de l'appareil respiratoire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Nucleic acid amplification techniques have improved the diagnostic possibilities in respiratory tract infections, although their clinical applicability is not yet fully defined. We have evaluated a multiplex real-time PCR method for the detection of 13 respiratory viruses and 2 bacteria (Mycoplasma and Chlamydophila) in a clinical setting. Objectives: The aim of the present study was to evaluate the diagnostic performance and clinical use of a novel multiplex PCR method in adults with community-acquired respiratory viral infection, and the impact of duration of symptoms on detection rates. Study design: Nasopharyngeal swab samples were prospectively collected from 209 adult outpatients with respiratory infections and 100 asymptomatic controls. Results: An infectious agent was identified in 43% of samples from patients and 2% of asymptomatic controls. The detection rate was significantly higher in samples from patients with a duration of symptoms of 6 days or less (51 %) than in samples from patients with a duration of symptoms of 7 days or more (30%, p < 0.01). For human corona viruses, and influenza virus A and B there was a correlation between the amount of virus in each patient sample as measured Ct values and duration of symptoms. Conclusions: Duration of symptoms significantly affects the detection rate of respiratory pathogens by multiplex real-time PCR in nasopharyngeal swab samples from adult patients with respiratory infections. 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Study design: Nasopharyngeal swab samples were prospectively collected from 209 adult outpatients with respiratory infections and 100 asymptomatic controls. Results: An infectious agent was identified in 43% of samples from patients and 2% of asymptomatic controls. The detection rate was significantly higher in samples from patients with a duration of symptoms of 6 days or less (51 %) than in samples from patients with a duration of symptoms of 7 days or more (30%, p < 0.01). For human corona viruses, and influenza virus A and B there was a correlation between the amount of virus in each patient sample as measured Ct values and duration of symptoms. Conclusions: Duration of symptoms significantly affects the detection rate of respiratory pathogens by multiplex real-time PCR in nasopharyngeal swab samples from adult patients with respiratory infections. 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